primary human lung microvascular endothelial cells hlmvecs  (PromoCell)

Journal: Frontiers in Cell and Developmental Biology

Article Title: Trauma promotes heparan sulfate modifications and cleavage that disrupt homeostatic gene expression in microvascular endothelial cells

doi: 10.3389/fcell.2024.1390794

Figure Lengend Snippet: Top differentially expressed genes in flow conditioned primary human lung microvascular endothelial cells (HLMVEC) following exposure to heparinase III (HepIII) relative to vehicle control. Messenger RNA was collected from confluent monolayers of HLMVEC that were conditioned with 15 dyn/cm 2 for 48 h followed by exposure to heparinase III 500 mU/mL or vehicle for 6 h while remaining under shear stress ( n = 4 biological replicates per condition; two replicates were pooled to generate two samples per condition for RNAseq). (A) Heatmap representing top 40 differentially expressed genes in HLMVEC between heparinase III and vehicle. Each treatment group contains n = 2 RNA samples that were combined from HLMVEC within two ibidi channel slides, thus representing a total of n = 4 per condition. Colors represent gene expression z-score with red corresponding to upregulated and blue to downregulated. (B) Volcano plot depicting differential gene expression between HLMVEC exposed to heparinase III (positive log2 fold change) and vehicle (negative log2 fold change). Red genes meet figure thresholds of p ≤ 1 × 10 −3 and log2 fold change ≥|1| for the purposes of visualization. (C) Expression of the flow-responsive genes Krüppel-like factor 2 and 4 ( KLF2 , 4 ), endothelial nitric oxide synthase ( NOS3 ) and solute carrier family nine isoform A3 regulatory factor 2 ( SLC9A3R2 ) is reduced following heparinase III treatment. (D) Expression of angiopoietin-2 ( ANGPT2 ), endothelial cell-specific molecule-1 ( ESM1 , also known as endocan), and thrombospondin ( THBS1 ), markers of endothelial cell activation, is increased following heparinase III treatment.

Article Snippet: Primary human lung microvascular ECs (HLMVEC) (S540-05a, Cell Applications, San Diego, CA, United States) were conditioned under shear stress (15 dyn/cm 2 ) for 48 h as described previously ( ).

Techniques: Control, Shear, Gene Expression, Expressing, Activation Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: Trauma promotes heparan sulfate modifications and cleavage that disrupt homeostatic gene expression in microvascular endothelial cells

doi: 10.3389/fcell.2024.1390794

Figure Lengend Snippet: Targeted representation of gene set enrichment analysis (GSEA) in flow conditioned (15 dyn/cm 2 for 48 h) primary human lung microvascular endothelial cells (HLMVEC) after 6-h exposure to vehicle or heparinase III (HepIII, 500 mU/mL) while remaining under shear stress ( n = 4 biological replicates per condition; two replicates were pooled to generate two samples per condition for RNAseq). GSEA was performed using (A) GO: Biological Process and (B) KEGG datasets. Figure displays up to twenty pathways from GSEA that are most relevant to endothelial cell organization and function with lowest False discovery rate (FDR)-adjusted p values (FDR q value). Pathways were organized according to their contribution to cellular maintenance and bioenergetics; cell organization and adhesion; angiogenesis and wound healing; or response to biophysical cues. Pathways on presented on the left were enriched in HLMVEC after exposure to vehicle whereas pathways on the right were enriched in HLMVEC after exposure to heparinase III. Circle size corresponds with number of genes present in experimental samples that overlap with respective dataset pathways, and circle shading represents the −log10 (FDR q value) with darker shades representing lower q values.

Article Snippet: Primary human lung microvascular ECs (HLMVEC) (S540-05a, Cell Applications, San Diego, CA, United States) were conditioned under shear stress (15 dyn/cm 2 ) for 48 h as described previously ( ).

Techniques: Shear

Journal: Frontiers in Cell and Developmental Biology

Article Title: Trauma promotes heparan sulfate modifications and cleavage that disrupt homeostatic gene expression in microvascular endothelial cells

doi: 10.3389/fcell.2024.1390794

Figure Lengend Snippet: Differentially expressed genes that govern synthesis of heparan sulfate proteoglycans and glycosaminoglycans in flow conditioned (15 dyn/cm 2 for 48 h) primary human lung microvascular endothelial cells treated for 6 h with vehicle or heparinase III (HepIII, 500 mU/mL) while remaining under shear stress ( n = 4 biological replicates per condition; two replicates were pooled to generate two samples per condition for RNAseq). (A) Of the heparan sulfate proteoglycans found in the vascular endothelial apical glycocalyx, expression of syndecan 3 ( SDC3 ) and SDC4 were downregulated by heparinase III treatment. (B) Of the enzymes regulating hyaluronan expression in the endothelial glycocalyx, hyaluronan synthase isoform 2 ( HAS2 ) was upregulated while hyaluronidases 1 and 2 ( HYAL1 , 2 ) were downregulated by heparinase III treatment. (C) Of the enzymes that synthesize chondroitin sulfate expressed in the endothelial glycocalyx (commonly observed in SDC1 and SDC3) and that modify its sulfation, chondroitin sulfate synthase isoform 3 ( CHSY3 ) and chondroitin sulfate N -acetylgalactosaminylsulfotransferase isoform 1 ( CSGALNACT1 ) were upregulated while carbohydrate sulfotransferase isoform 15 ( CHST15 , catalyzing 6- O -sulfation of 4- O -sulfated N -acetylgalactosamine in chondroitin sulfate disaccharides) was downregulated following heparinase III treatment. (D) Of the enzymes that synthesize and modify heparan sulfate expressed in the endothelial glycocalyx, expression of N -deacetylase/ N -sulfotransferase isoform 1 ( NDST1 ) and glucuronic acid C5-epimerase ( GLCE ) (which also contributes to glucuronic acid epimerization to iduronic acid in chondroitin sulfate) were downregulated while heparan sulfate 3- O -sulfotransferase isoform 1 ( HS3ST1 ) and heparan sulfate 6- O -sulfotransferase isoform 3 ( HS6ST3 ) were upregulated following heparinase III treatment. We also found that heparinase III treatment suppressed heparanase ( HPSE ) expression. False discovery rate (FDR)-adjusted p values (FDR q values) are presented.

Article Snippet: Primary human lung microvascular ECs (HLMVEC) (S540-05a, Cell Applications, San Diego, CA, United States) were conditioned under shear stress (15 dyn/cm 2 ) for 48 h as described previously ( ).

Techniques: Shear, Expressing, Histone Deacetylase Assay

Journal: Frontiers in Immunology

Article Title: Protective role of Cav-1 in pneumolysin-induced endothelial barrier dysfunction

doi: 10.3389/fimmu.2022.945656

Figure Lengend Snippet: Caveolin-1 overexpression protects against Pneumolysin (PLY) induced calcium entry and endothelial barrier dysfunction. (A) Caveolin-1 overexpression or Cav-1 peptide blocks PLY induced Ca 2+ entry in Cos7 cells. Data are represented as mean ± SE (n=3-4), * P<0.05, **P<0.01 vs. vehicle control. (B) HLMVEC cells were transduced with 10-10 MOI RFP (RFP ad.) or caveolin-1 adenovirus (Cav-1 ad.) ( Inset : western blot analysis of Caveolin-1 overexpression. Hsp90 was used as loading control), or (C) treated with 10 μM caveolin-1 scaffolding domain peptide for 24 hours, and TER of vehicle or PLY (60ng/mL) treated HLMVECs was measured (n=3). ***P<0.001, ****P<0.0001. One-way ANOVA (with Tukey’s post-hoc test).

Article Snippet: Primary Human Lung Microvascular Endothelial Cells (HLMVECs) were purchased from Lonza and grown in Endothelial Growth Medium-2-Microvessel (EGM-2MV) containing the requisite growth factors and 5% (FBS) (Lonza, Allendale, NJ).

Techniques: Over Expression, Control, Transduction, Western Blot, Scaffolding

Journal: Frontiers in Immunology

Article Title: Protective role of Cav-1 in pneumolysin-induced endothelial barrier dysfunction

doi: 10.3389/fimmu.2022.945656

Figure Lengend Snippet: Knock-out or silencing of Cav-1 facilitates PLY induced barrier dysfunction in human lung microvascular endothelial cells. (A) Control and Cav-1 knockout HULEC5α cells were treated with PLY and barrier function assessed by changes in TER. (n=3). **P<0.01 control-PLY vs Cav-1 KO-PLY, # P<0.0001 when compared to vehicle control. (B) HLMVECs were treated with non-targeting (nsRNA) or caveolin-1 specific silencing RNA (siCav-1) for 72 hours and TER was measured upon challenge with PLY (60 ng/mL), (n=3-4). ****P<0.0001 control-PLY vs siCav-1-PLY, # P<0.0001 when compared to vehicle control. Two-way ANOVA (with Tukey’s multiple comparisons test).

Article Snippet: Primary Human Lung Microvascular Endothelial Cells (HLMVECs) were purchased from Lonza and grown in Endothelial Growth Medium-2-Microvessel (EGM-2MV) containing the requisite growth factors and 5% (FBS) (Lonza, Allendale, NJ).

Techniques: Knock-Out, Control

Journal: Frontiers in Immunology

Article Title: Protective role of Cav-1 in pneumolysin-induced endothelial barrier dysfunction

doi: 10.3389/fimmu.2022.945656

Figure Lengend Snippet: Reconstitution of Cav-1 or its scaffolding domain signaling attenuates PLY-induced endothelial barrier disruption. (A) Caveolin-1 overexpression in Cav-1 knock-out HULEC5α cells ameliorates PLY induced endothelial barrier dysfunction. Cav-1 knock-out endothelial cells were transduced with RFP or Cav-1 adenovirus (10 MOI). After 48 hours the cells were treated with either vehicle or PLY (60ng/mL) and barrier function assessed by changes in TER. (B) Caveolin-1 scaffolding domain peptide ameliorates PLY induced endothelial barrier dysfunction. Cav-1 knockout HULEC5α were administered the Cav-1 scaffolding domain peptide (10 µM) or vehicle (DMSO). After 24 hours, cells were treated with vehicle (Veh.) or PLY (60 ng/mL) and TER was measured. (C) HLMVECs were transfected with non-specific or Cav-1 specific silencing RNA and rescue experiments were performed by transducing Cav-1 silenced cells with RFP or Cav-1 adenovirus. After 24 hours HLMVECs were treated with vehicle (Veh) or PLY (60 ng/mL) and TER was measured. (D) HLMVECs were transfected with non-specific or Cav-1 specific silencing RNA and rescue experiments were performed by treating the cells with Cav-1 scaffolding peptide (10 µM) or DMSO for 24 hours, followed by PLY (60 ng/mL). Endothelial barrier function was assessed by changes in TER (n=3). ****P<0.0001, two-way ANOVA (with Tukey’s multiple comparisons test).

Article Snippet: Primary Human Lung Microvascular Endothelial Cells (HLMVECs) were purchased from Lonza and grown in Endothelial Growth Medium-2-Microvessel (EGM-2MV) containing the requisite growth factors and 5% (FBS) (Lonza, Allendale, NJ).

Techniques: Scaffolding, Disruption, Over Expression, Knock-Out, Transduction, Transfection